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Wayne Barnes
Associate Professor
Dept. of Biochemistry and
Molecular Biophysics |
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Mailing Address:
WUSM - Biochemistry Dept.
660 S. Euclid Ave., MS8231
St. Louis, MO 63110
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Research Interests
PCR methods; plant genetic engineering for insect or virus resistance
Selected Publications
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Kermekchiev, M.B., Kirilova, L.I., Vail, E.E. and Barnes, W. Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples. Nucleic Acids Res. 37:e40 (2009).
Ren, C.P., Chaudhuri, R.R., Fivian, A., Bailey, C.M., Antonio, M., Barnes, W.M. and Pallen, M.J. The ETT2 gene cluster, encoding a second type III secretion system from Escherichia coli, is present in the majority of strains but has undergone widespread mutational attrition. J Bacteriology 186:3547-3560 (2004).
Kermekchiew, M.B. Tzekov, A. and Barnes, W.M. Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. Nucleic Acids research 31:6139-6147 (2003).
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Stratman, J.L., Barnes, W.M. and Simon, T.C. Universal PCR genotyping assay that achieve single copy sensitivity with any primer pair. Transgenic Research 12:521-522 (2003).
