©2001
by the Department of
Biochemistry and
Molecular Biophysics
at Washington
University School of
Medicine

Carl Frieden

Position available to study the mechanism and dynamics of protein folding. Methods used include labeling proteins with fluoro amino acids and following folding in real time with NMR allowing measurements of side chain packing and/or stabilization during folding. Candidates should have a background in NMR. Knowldege of site-directed mutagenesis would be useful. Requires Ph.D. or equivalent degree.

Send C.V. with names of three references to frieden@biochem.wustl.edu or to:

Dr. Carl Frieden
Department of Biochemistry and Molecular Biophysics
Washington University School of Medicine
Box 8231, 660 S. Euclid Ave.
St. Louis, MO 63110

William A. Frazier - Cell and Molecular Biology of Integrin Regulation

A postdoctoral position is currently available to study the functional interactions of CD47/integrin associated protein with its ligands, thrombospondin and SIRPalpha, with its co-receptors, the integrins and with their associated signaling molecules. We have defined these interactions biochemically and have developed panels of cell lines deficient in CD47 or particular integrins. These will be used for expression of fluorescent protein fusions of CD47, integrins and signaling proteins to study the localization and interactions of these components in living cells using fluorescence imaging techniques. Of particular interest is the role of CD47 in integrin-mediated cell motility (Brown and Frazier, Trends Cell Biol 11:130, 2001) and in programmed cell death (Manna and Frazier, J. Immunol. 170:3544, 2003). A background in microscopy of livng cells, fluorescence methods and molecular biology is required. Start up funding is available for this position with the expectation that candidates will be qualified for fellowships from national funding agencies such as NIH, AHA, etc.

Interested candidates should reply via email to frazier@biochem.wustl.edu . Please attach a current CV and a brief statement of research background and interests. A good command of written and spoken English is required.

Victor Guallar

A vacancy exists for one postdoctoral researcher. Candidates must have a Ph.D. (or equivalent), a strong research background in computational modeling, and a good command of written and spoken English.

Interested applicants should reply via e-mail to: guallarv@biochem.wustl.edu. Please attach a current CV with the names of at least three references and a brief statement of research background and interests.

Postdoctoral positions are available for studies of DNA motor proteins (helicases) involved in DNA repair and SSB protein-DNA interactions using biochemical, biophysical and mutational approaches. Particular emphasis is on mechanistic studies using transient kinetic methods (fluorescence, stopped-flow, quenched-flow) as well as thermodynamic (equilibrium binding and isothermal titration calorimetry, analytical ultracentrifugation) and structural (x-ray crystallography) approaches. Please see my home page.

Dr. Timothy M. Lohman
Washington University School of Medicine
Dept. of Biochemistry & Molecular Biophysics
660 S. Euclid Avenue
Campus Box 8231
St. Louis, MO 63110

Or send e-mail to: Dr. Timothy M. Lohman